藤田医科大学医学部生化学講座（原田名誉教授）
»現在の生化学講座の業績（2019年から）はこちらをご参照ください。
２０１９年
Fujii R, Yamada H, Munetsuna E, Yamazaki M, Mizuno G, Tsuboi Y, Ohashi K,
Ishikawa H, Ando Y, Hagiwara C, Maeda K, Hashimoto S, Hamajima N, Suzuki K.
Dietary vegetable intake is inversely associated with ATP-binding cassette
protein A1 (ABCA1) DNA methylation levels among Japanese women. Nutrition. 2019;65:1-5. doi: 10.1016/j.nut.2019.02.010.
Fujii R, Yamada H, Munetsuna E, Yamazaki M, Ohashi K, Ishikawa H, Maeda K,
Hagiwara C, Ando Y, Hashimoto S, Hamajima N, Suzuki K. Associations of
circulating microRNAs (miR-17, miR-21, and miR-150) and chronic kidney disease in
a Japanese population. J Epidemiol. 2019 Mar 23. doi: 10.2188/jea.JE20180233.
[Epub ahead of print]
Munetsuna E, Yamada H, Yamazaki M, Ando Y, Mizuno G, Hattori Y, Sadamoto N,
Ishikawa H, Ohta Y, Fujii R, Suzuki K, Hashimoto S, Ohashi K. Maternal
high-fructose intake increases circulating corticosterone levels via decreased
adrenal corticosterone clearance in adult offspring. J Nutr Biochem. 2019;67:44-50. doi: 10.1016/j.jnutbio.2019.01.016.
Kondo M, Yamada H, Munetsuna E, Yamazaki M, Hatta T, Iwahara A, Ohashi K,
Ishikawa H, Tsuboi Y, Inoue T, Fujii R, Suzuki K. Associations of serum
microRNA-20a, -27a, and -103a with cognitive function in a Japanese population:
The Yakumo study. Arch Gerontol Geriatr. 2019;82:155-160. doi:
10.1016/j.archger.2019.01.007.
Ishikawa H, Yamada H, Kondo K, Ota T, Yamazaki M, Ando Y, Mizuno G, Munetsuna E, Suzuki K, Teradaira R, Ohashi K. Establishment of a simpler method for
measuring HDL-microRNAs. Ann Clin Biochem. 2019;56:49-55. doi:
10.1177/0004563218775770.
２０１8年
Diano S, Horvath TL, Mor G, Register T, Adams M, Harada N, Naftolin F.
Aromatase and estrogen receptor immunoreactivity in the coronary arteries of
monkeys and human subjects. Menopause. 2018; 25:1201-1207. doi:
10.1097/GME.0000000000001219.
Ohe K, Miyajima S, Tanaka T, Hamaguchi Y, Harada Y, Horita Y, Beppu Y, Ito F,
Yamasaki T, Terai H, Mori M, Murata Y, Tanabe M, Abe I, Ashida K, Kobayashi K,
Enjoji M, Nomiyama T, Yanase T, Harada N, Utsumi T, Mayeda A. HMGA1a Induces
Alternative Splicing of the Estrogen Receptor-αlpha Gene by Trapping U1 snRNP to
an Upstream Pseudo-5' Splice Site. Front Mol Biosci. 2018;5:52. doi:
10.3389/fmolb.2018.00052.
Hayashi T, Hikichi M, Yukitake J, Wakatsuki T, Nishio E, Utsumi T, Harada N.
Forskolin increases the effect of everolimus on aromatase inhibitor-resistant
breast cancer cells. Oncotarget. 2018;9:23451-23461. doi:
10.18632/oncotarget.25217.
Ohe K, Miyajima S, Abe I, Tanaka T, Hamaguchi Y, Harada Y, Horita Y, Beppu Y,
Ito F, Yamasaki T, Terai H, Mori M, Murata Y, Tanabe M, Ashida K, Kobayashi K,
Enjoji M, Yanase T, Harada N, Utsumi T, Mayeda A. HMGA1a induces alternative
splicing of estrogen receptor alpha in MCF-7 human breast cancer cells. J Steroid
Biochem Mol Biol. 2018;182:21-26. doi: 10.1016/j.jsbmb.2018.04.007.
Munetsuna E, Yamada H, Yamazaki M, Ando Y, Mizuno G, Ota T, Hattori Y,
Sadamoto N, Suzuki K, Ishikawa H, Hashimoto S, Ohashi K. Maternal fructose intake
disturbs ovarian estradiol synthesis in rats. Life Sci. 2018;202:117-123.
doi: 10.1016/j.lfs.2018.04.006.
Tsuboi Y, Yamada H, Munetsuna E, Yamazaki M, Mizuno G, Murase Y, Ohashi K,
Ishikawa H, Kondo M, Inoue T, Hashimoto S, Hamajima N, Suzuki K. Relationship
between Long Interspersed Nuclear Element-1 DNA Methylation in Leukocytes and
Dyslipidemia in the Japanese General Population. J Atheroscler Thromb. 2018;25:1231-1239. doi: 10.5551/jat.43570.
Yamazaki M, Yamada H, Munetsuna E, Ishikawa H, Mizuno G, Mukuda T, Mouri A,
Nabeshima T, Saito K, Suzuki K, Hashimoto S, Ohashi K. Excess maternal fructose
consumption impairs hippocampal function in offspring via epigenetic modification
of BDNF promoter. FASEB J. 2018;32:2549-2562. doi: 10.1096/fj.201700783RR.
Munetsuna E, Yamada H, Ando Y, Yamazaki M, Tsuboi Y, Kondo M, Mizuno G,
Ishikawa H, Sugimoto K, Osakabe K, Ichino N, Ohashi K, Hamajima N, Suzuki K.
Association of subcutaneous and visceral fat with circulating microRNAs in a
middle-aged Japanese population. Ann Clin Biochem. 2018;55:437-445. doi:
10.1177/0004563217735124.
２０１7年
Hayashi T, Hikichi M, Yukitake J, Harada N, Utsumi T. Estradiol suppresses
phosphorylation of ERα serine 167 through upregulation of PP2A in breast cancer
cells. Oncol Lett. 2017 Dec;14(6):8060-8065. doi: 10.3892/ol.2017.7216. Epub 2017
Oct 18. PubMed PMID: 29344249; PubMed Central PMCID: PMC5755173.
Honma N, Saji S, Mikami T, Yoshimura N, Mori S, Saito Y, Murayama S, Harada N.
Estrogen-Related Factors in the Frontal Lobe of Alzheimer's Disease Patients and
Importance of Body Mass Index. Sci Rep. 2017;7:726. doi:
10.1038/s41598-017-00815-3.
Ishihara S, Kotomura N, Yamamoto N, Ochiai H. Ligation-mediated PCR with a
back-to-back adapter reduces amplification bias resulting from variations in GC
content. Anal Biochem. 2017;531:37-44. doi: 10.1016/j.ab.2017.05.011.
Mizuno G, Munetsuna E, Yamada H, Ando Y, Yamazaki M, Murase Y, Kondo K,
Ishikawa H, Teradaira R, Suzuki K, Ohashi K. Fructose intake during gestation and
lactation differentially affects the expression of hippocampal neurosteroidogenic
enzymes in rat offspring. Endocr Res. 2017;42:71-77. doi:
10.1080/07435800.2016.1182186.
Ishikawa H, Yamada H, Taromaru N, Kondo K, Nagura A, Yamazaki M, Ando Y,
Munetsuna E, Suzuki K, Ohashi K, Teradaira R. Stability of serum high-density
lipoprotein-microRNAs for preanalytical conditions. Ann Clin Biochem. 2017;54:134-142. doi: 10.1177/0004563216647086.
２０１6年
Hiratsuka I, Yamada H, Munetsuna E, Hashimoto S, Itoh M. Circulating
MicroRNAs in Graves' Disease in Relation to Clinical Activity. Thyroid. 2016;26:1431-1440.
Yamazaki M, Munetsuna E, Yamada H, Ando Y, Mizuno G, Murase Y, Kondo K,
Ishikawa H, Teradaira R, Suzuki K, Ohashi K. Fructose consumption induces
hypomethylation of hepatic mitochondrial DNA in rats. Life Sci. 2016;149:146-52. doi: 10.1016/j.lfs.2016.02.020.
２０１5年
Kotomura N, Harada N, Ishihara S. The Proportion of Chromatin Graded between
Closed and Open States Determines the Level of Transcripts Derived from Distinct
Promoters in the CYP19 Gene. PLoS One. 2015 May;10:e0128282. doi:
10.1371/journal.pone.0128282.
Kurokawa J, Sasano T, Kodama M, Li M, Ebana Y, Harada N, Honda S, Nakaya H,
Furukawa T. Aromatase knockout mice reveal an impact of estrogen on drug-induced
alternation of murine electrocardiography parameters. J Toxicol Sci. 2015
Jun;40:339-48. doi: 10.2131/jts.40.339.
Iwasa A, Arakaki R, Honma N, Ushio A, Yamada A, Kondo T, Kurosawa E,
Kujiraoka S, Tsunematsu T, Kudo Y, Tanaka E, Yoshimura N, Harada N, Hayashi Y,
Ishimaru N. Aromatase controls Sjögren syndrome-like lesions through monocyte
chemotactic protein-1 in target organ and adipose tissue-associated macrophages.
Am J Pathol. 2015;185:151-61. doi: 10.1016/j.ajpath.2014.09.006.
Munetsuna E, Kittaka A, Chen TC, Sakaki T. Metabolism and Action of
25-Hydroxy-19-nor-Vitamin D₃ in Human Prostate Cells. Vitam Horm.
2016;100:357-77. doi: 10.1016/bs.vh.2015.10.009.
Ohashi K, Munetsuna E, Yamada H, Ando Y, Yamazaki M, Taromaru N, Nagura A,
Ishikawa H, Suzuki K, Teradaira R, Hashimoto S. High fructose consumption induces
DNA methylation at PPARα and CPT1A promoter regions in the rat liver. Biochem
Biophys Res Commun. 2015;468:185-9. doi:
10.1016/j.bbrc.2015.10.134.
Yamada H, Ohashi K, Suzuki K, Munetsuna E, Ando Y, Yamazaki M, Ishikawa H,
Ichino N, Teradaira R, Hashimoto S. Longitudinal study of circulating miR-122 in
a rat model of non-alcoholic fatty liver disease. Clin Chim Acta. 2015;446:267-71. doi: 10.1016/j.cca.2015.05.002.
２０１4年
Hayashi T, Harada N. Post-translational dual regulation of cytochrome P450
aromatase at the catalytic and protein levels by
phosphorylation/dephosphorylation. FEBS J. 2014;281:4830-40. doi:
10.1111/febs.13021.
Lai WA, Yeh YT, Fang WL, Wu LS, Harada N, Wang PH, Ke FC, Lee WL, Hwang JJ.
Calcineurin and CRTC2 mediate FSH and TGFβ1 upregulation of Cyp19a1 and Nr5a in
ovary granulosa cells. J Mol Endocrinol. 2014;53:259-70. doi:
10.1530/JME-14-0048.
Hoyk Z, Csakvari E, Gyenes A, Siklos L, Harada N, Parducz A. Aromatase and
estrogen receptor beta expression in the rat olfactory bulb: Neuroestrogen action
in the first relay station of the olfactory pathway? Acta Neurobiol Exp (Wars).
2014;74:1-14.
Ubuka T, Haraguchi S, Tobari Y, Narihiro M, Ishikawa K, Hayashi T, Harada N,
Tsutsui K. Hypothalamic inhibition of socio-sexual behaviour by increasing
neuroestrogen synthesis. Nat Commun. 2014;5:3061. doi: 10.1038/ncomms4061.
Ohashi K, Ando Y, Munetsuna E, Yamada H, Yamazaki M, Nagura A, Taromaru N,
Ishikawa H, Suzuki K, Teradaira R. Maternal fructose consumption alters messenger
RNA expression of hippocampal StAR, PBR, P450(11β), 11β-HSD, and 17β-HSD in rat
offspring. Nutr Res. 2015;35:259-64. doi: 10.1016/j.nutres.2014.11.001.
Munetsuna E, Kawanami R, Nishikawa M, Ikeda S, Nakabayashi S, Yasuda K, Ohta
M, Kamakura M, Ikushiro S, Sakaki T. Anti-proliferative activity of
25-hydroxyvitamin D3 in human prostate cells. Mol Cell Endocrinol. 2014;382:960-70. doi: 10.1016/j.mce.2013.11.014.
２０１３年以前
Honda S, Wakatsuki T, Harada N. Behavioral analysis of genetically modified
mice indicates essential roles of neurosteroidal estrogen. Front Endocrinol
(Lausanne). 2011;2:40. doi: 10.3389/fendo.2011.00040.
Kawakami M, Harada N, Hiratsuka M, Kawai K, Nakamura Y. Dietary
isothiocyanates modify mitochondrial functions through their electrophilic
reaction. Biosci Biotechnol Biochem. 2005;69:2439-44.
Harada N, Honda S. Analysis of spatiotemporal regulation of aromatase in the
brain using transgenic mice. J Steroid Biochem Mol Biol. 2005;95:49-55.
Yoshimura N, Harada N, Bukholm I, Kåresen R, Børresen-Dale AL, Kristensen VN.
Intratumoural mRNA expression of genes from the oestradiol metabolic pathway and
clinical and histopathological parameters of breast cancer. Breast Cancer Res.
2004;6:R46-55.
Harada N, Yoshimura N, Honda S. Unique regulation of expression of human
aromatase in the placenta. J Steroid Biochem Mol Biol. 2003;86:327-34.
Review.
Matsumoto T, Honda S, Harada N. Alteration in sex-specific behaviors in male
mice lacking the aromatase gene. Neuroendocrinology. 2003 Jun;77(6):416-24.
PubMed PMID: 12845227.
Kato S, Mano T, Kobayashi T, Yamazaki N, Himeno Y, Yamamoto K, Itoh M, Harada N, Nagasaka A. A calcium-deficient diet caused decreased bone mineral density and
secondary elevation of estrogen in aged male rats-effect of menatetrenone and
elcatonin. Metabolism. 2002;51:1230-4.
