T-cel Data

Research Title	Gene profiling analysis of allergen specific T cells in henfs egg allergy.
Investigator	Yoichi Nakajima(Fujita Health University ,Department of Pediatrics)
Summary	Although changes in the fine balance of allergen-specific T cells are crucial in the pathogenesis of allergic diseases, their roles in the allergic reaction to hen's eggs (HE) have not yet been fully analyzed. By using microarray technology, allergen-stimulated T cells from HE-allergic children were analyzed to identify genes that are specifically up-regulated in these cells. RNA from allergen-stimulated CD4+ CD14- cells was analyzed using whole genome microarray. For each sample, RNA of non-stimulated peripheral blood mononuclear cells (PBMCs) and RNA of allergen-stimulated PBMCs were labeled with Cy3 and Cy5, respectively. Hybridizations on the microarray slide of the Human Whole Genome (Agilent Technologies), which contains about 44000 sequenced human genes, were carried out for 17 hours in a rotating hybridization oven using an In Situ Hybridization kit Plus. The slide was washed as indicated in the protocol and was then scanned with an Agilent G2565BA microarray scanner.
Organism	Homo sapiens
Data Description
Data (Downloadable) Help	Hen's egg allergic patient1 Hen's egg allergic patient2 Hen's egg allergic patient3 Non hen's egg allergic child1 Non hen's egg allergic child2
Experimental Design
GEO Series Accession No.	GSE7512